规格
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Assay Entry:
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Cell-based beta lactamase reporter gene |
Cell State:
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Dividing Cells |
Druggable Target:
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Signaling Pathway |
Product Line:
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CellSensor™ |
System Type:
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CellSensor™ |
Target Entry:
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PKA, cAMP, cAMP/PKA pathway |
储存
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CellSensor® Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use. |
The CellSensor® CRE-bla HEK 293T cell line contains the b-lactamase reporter gene under control of the cAMP response element (CRE). To obtain the cell line, the CRE-bla construct was transduced into HEK 293T cells by lentivirus. Subsequent flow cytometry was used to isolate clones responsive to forskolin stimulation. The CellSensor® CRE-bla HEK 293T cell line can be used to build specific G-protein coupled receptor (GPCR) assays or to detect changes in intracellular cAMP levels. Academic and non-profit customers, please inquire for special pricing.