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FLUO-4 DIRECT CALCIUM ASSAY KI 1 KIT,F10473,Invitrogen

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订货号 1JC1837
品牌型号 Invitrogen F10473
货期 询货期
最小订货量 1套
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产品介绍 Product Description
规格
Cell Permeability: Cell-Permeant
Emission: Visible
Excitation: Visible
Excitation/Emission: 488⁄530
Form: Powder
Indicator: Calcium Indicator
Indicator Properties: Fluorescent Dye-Based
Modification: Chemical
Product Line: Molecular Probes®
Quantity: 1 x 1L
Shipping Condition: Room Temperature
Sub Cellular Localization: Nucleus, Organelles, Cytoplasm
Target Function: Signal Transduction
Volume (Metric): 1000 ml
Detection Method: Fluorescent
For Use With (Equipment): Confocal Microscope, Fluorescence Microscope, High Content Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
Color: Green
储存
• 1 L Fluo-4 Direct™ Calcium Assay Reagent (Component A)
• 1.54 g Probenecid (Component B)

Store at ≤-20°C. Dessicate and protect from light.
描述

The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that:
1. Easily loads into cells
2. Achieves a large assay window and
3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescence

This advanced formulation allows the assay to be run in a simple “addition only" format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.

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