规格
|
Endotoxin Level:
|
< 1 EU/µg |
Purity or Quality Grade:
|
Carrier-Free |
Quantity:
|
100 μg |
Shipping Condition:
|
Approved for shipment on Wet or Dry Ice |
Expression System:
|
E. coli |
Protein Family:
|
Growth Factors & Receptors |
Research Category:
|
Neurobiology, Oncology, Stem Cell Research, Microbiology, Differentiation, Angiogenesis |
Species:
|
Human |
Validated Application:
|
Cell Culture |
Molecular Weight:
|
19 kDa |
Purity:
|
> 95% by SDS-PAGE |
Protein Length:
|
155 aa + 20 aa for N-terminal tag for purification purposes |
Biological Activity:
|
ED50 range = 0.6-1.1 ng⁄mL |
Sterility:
|
Filtered before lyophilization through a 0.22 micron sterile filter. |
储存
|
Store the lyophilized protein at −20°C, preferably desiccated. |
Heat Stable Recombinant Human bFGF (also known as fibroblast growth factor-basic, FGF-basic, FGF-β, or FGF2) is a full-length protein (containing 155 amino acid residues) that has been engineered for greater stability in cell culture conditions. It sustains ≥80% activity at 37°C for at least 72 hours compared to native bFGF, which has a half-life of <8 hours at 37°C.
• Superior performance—retains bioactivity without the use of artificially high concentrations
• Improved efficiency—the stable bioactivity of Heat Stable bFGF means you won’t have to supplement with additional bFGF (a necessary step when using native bFGF), and you’ll save money in bFGF reagent costs over the longer term
• Easy to use—direct replacement for your current bFGF, allowing for easy substitution in protocols
• Advancement without compromise—>90% sequence homology to native bFGF, leaving heparin and FGF receptor binding sites untouched