规格
|
Binding Property:
|
Standard Capacity, Affinity |
Plate Blocking:
|
SuperBlock™ Buffer |
Plate Type:
|
Detection Plate, Immunoassay, ELISA |
Product Line:
|
NeutrAvidin™, Pierce™ |
Color:
|
Clear |
Detection Method:
|
Colorimetric |
Format:
|
384-well plate |
Surface Coating:
|
NeutrAvidin™ Protein |
储存
|
Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C. Plates are shipped at ambient temperature. |
Thermo Scientific NeutrAvidin Protein Coated White 96-Well Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.
Features of NeutrAvidin Protein Coated White 96-Well Plates:
• White plates for chemiluminescent detection methods
• Pre-blocked with Blocker BSA Buffer
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Less expensive than streptavidin coated plates
• Lowest nonspecific binding properties of all biotin-binding proteins
Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.
Related Products
Pierce™ NeutrAvidin™ Coated Plates, Black, 96-Well
Pierce™ NeutrAvidin™ Coated Plates, White, 384-Well
Pierce™ NeutrAvidin™ Coated Plates, Black, 384-Well