规格
|
Cell Permeability:
|
Cell-Impermeant |
Detection Method:
|
Fluorescent |
For Use With (Equipment):
|
Microplate Reader |
Indicator:
|
pH Indicator |
Indicator Properties:
|
Fluorescent Dye-Based |
Shipping Condition:
|
Room Temperature |
储存
|
Store at room temperature and protect from light. |
BCECF is ideal for measuring changes ± 1 pH unit around pH 7, because it is a dual-excitation ratiometric pH indicator with a pKa of ~6.98. pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus the emission intensity when excited at its isobestic point of ~440 nm.