- 商品介绍
- 规格参数
- 包装参数
规格 |
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Final Product Type: | Total RNA |
For Use With (Application): | Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction |
Purification Target: | Total RNA |
Sample Type: | Tissue, Cells |
Includes: | Formaldehyde Load Dye, Lysis/Binding Solution, Water for 64% Ethanol, Lithium, Chloride Solution, Wash Solution No. 1 and Wash Solution 2/3 Concentrate, Syringe Filter Units, Collection Tubes and Elution Solution |
Isolation Technology: | Syringe Filter (Glass Fiber) |
Product Type: | Kit |
High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
Shipping Condition: | Room Temperature |
Product Line: | Ambion™, RNAqueous® |
储存 |
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Formaldehyde Load Dye should bestored at -20°C; Lysis/Binding Solution, Water for 64% Ethanol, LithiumChloride Solution, Wash Solution #1 and Wash Solution 2/3 Concentrate should bestored at 4°C; Syringe Filter Units, Collection Tubes and Elution Solution canbe stored at room temperature. All reagents are guaranteed stable for 6 months whenproperly stored. |
描述 |
The Ambion® RNAqueous®-Midi Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 107-108 cells using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. The RNAqueous®-Midi Kit provides enough reagents for 15 RNA purifications.
• Convenient syringe-based filtration
• Compatible with vacuum manifolds when processing multiple samples The Ambion® RNAqueous®-Midi Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
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