StraightFrom Leukopak CD4/CD8 MB Kit，130-122-352，美天旎

The StraightFrom^® Leukopak^® CD4/CD8 MicroBead Kit has been developed for the rapid simultaneous positive selection of CD4⁺ and CD8⁺ cells directly from a ½ Leukopak^® using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS^® Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Multi-24 Column Blocks and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of a ½ Leukopak^®.

The CD4 antigen is expressed on T helper (Tʜ) cells and at a lower level on monocytes and dendritic cells. The CD4 antigen is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimer on CD4⁺ Tʜ cells. The CD8 antigen is expressed on cytotoxic T cells and on a subset of CD16⁺ NK cells. The CD8 antigen acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8⁺ cytotoxic T cells.

A ½ Leukopak^® is labeled with StraightFrom^® Leukopak^® CD4/CD8 MicroBeads and loaded onto Whole Blood Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS^® Separator. After removing the column from the magnetic field, the magnetically retained CD4⁺ and CD8⁺ cells can be eluted as the positively selected cell fraction.

The purified CD4⁺ and CD8⁺ cells are well suited for further flow cytometric, functional, or molecular analysis.