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The Naive CD8a+ T Cell Isolation Kit was developed for the isolation of untouched naive CD8a+ T cells from mouse spleen and lymph node cells.
For magnetic depletion of memory CD8+ T cells and non-CD8+ T cells, single cell suspension is incubated with a cocktail of biotinylated CD45R, CD4, CD11b, CD11c, CD19, CD44, CD49b, CD105, Ter-119, MHC II, and anti-TCRγ/δ antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion.
Naive CD8a+ T cells can be used for further in vitro analysis of T cell differentiation or adoptive transfer experiments.
LS, XS, or autoMACS® Columns.
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