规格
|
Column Type:
|
Agarose Resin, Affinity |
Purification Target:
|
HA-tagged Proteins |
Stationary Phase:
|
Antibody |
Format:
|
Bottle(s) |
Primary or Secondary:
|
Primary |
储存
|
5 mL of settle resin, provided as 10 mL of 50% slurry. Store at 2–8°C. |
Lyophilized peptide with the sequence N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C. Molecular weight: 1102.2 g/mol
26184 can be used to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin. Use 4 X 1 column volumes of 1 mg/ml HA peptide solution to elute HA-tagged proteins. Elution yields will increase by incubating the peptide on the column for 10-15 minutes at 30-37ºC for each elution fraction.
Reconstitute to 1 mg/ml using TBS or distilled water.
The HA peptide, NH2-YPYDVPDYA-COOH, is a synthetic peptide used to competitively elute fusion proteins containing the influenza hemagglutinin (HA) tag bound to immobilized anti-HA affinity resins.