NUTRIENT MIXTURE F-10 W/HEPES 20X100ML，22390082，Invitrogen

规格
Classification:	Animal Origin-Free
Culture Environment:	CO2
HEPES Buffer:	HEPES
Osmolality:	270 - 320 mOsm/kg
Product Line:	Gibco®
Sodium Bicarbonate Buffer:	Sodium Bicarbonate
Sodium Pyruvate Additive:	Sodium Pyruvate
Supplementation Required:	Serum
Volume (Metric):	100 ml
Form:	Liquid
Glutamine:	L-Glutamine
Phenol Red Indicator:	Phenol Red
pH Range:	7.0 - 7.4
Concentration:	1 X

储存

Storage conditions: 2–8°C. Protect from light. Shipping conditions: Ambient. Shelf life: 12 months from date of manufacture.

描述

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.

The complete formulation is available.

Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).

cGMP Manufacturing and Quality System
Gibco® F-10 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® F-10 product made in our Grand Island facility (12390-035). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Gibco® F-10 contains no proteins or growth factors. Therefore, F-10 often requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L), and therefore requires a 5-10% CO₂ environment to maintain physiological pH.