Waymouth's Medium is a chemically defined medium developed by Charity Waymouth and originally formulated for studies on the nutrition, metabolism, and growth of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment. Gibco® Waymouth's Medium is also used for the culture of whole organs and a broad range of cell types, such as carcinoma cell lines.

This Waymouth's Medium is modified as follows:

With	Without
• L-glutamine	• HEPES
• Phenol Red

The complete formulation is available.

Dual-Site cGMP Manufacturing
Gibco® Waymouth's Medium is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® Waymouth's Medium product made in our Grand Island facility (11220-035). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Waymouth's Medium contains no proteins, lipids, or growth factors. Some cell types may required the supplementation with Fetal Bovine Serum (FBS). Waymouth's Medium uses a sodium bicarbonate buffer system (2.24 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

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