MEM (10X) 10X500ML，21430079，Invitrogen

规格
Classification:	Animal Origin-Free
Culture Environment:	CO2
HEPES Buffer:	No HEPES
Osmolality:	2400 - 3000 mOsm/kg
Product Line:	Gibco®
Serum Supplementation:	Standard Serum Supplementation
Sodium Bicarbonate Buffer:	No Sodium Bicarbonate
Sodium Pyruvate Additive:	No Sodium Pyruvate
Supplementation Required:	Serum, Sodium Bicarbonate
Volume (Metric):	500 ml
Form:	Liquid
Glutamine:	No Glutamine
Phenol Red Indicator:	Phenol Red
pH Range:	4.8 - 5.3, 1.2 - 1.8
Concentration:	10 X

储存

Storage conditions: 2-8° C. Protect from light. Shipping conditions: Ambient. Shelf life: 12 months from date of manufacture.

描述

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

The complete formulation is available.

Gibco® MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® MEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® MEM product made in our Grand Island facility (11430-030). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Concentrated forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).