MEM ALPHA W/NUCLEOSIDES 10X500ML，22571038，Invitrogen

规格
Classification:	Animal Origin-Free
Culture Environment:	CO2
HEPES Buffer:	No HEPES
Osmolality:	280 - 310 mOsm/kg
Product Line:	Gibco®
Serum Supplementation:	Standard Serum Supplementation
Sodium Bicarbonate Buffer:	Sodium Bicarbonate
Sodium Pyruvate Additive:	Sodium Pyruvate
Supplementation Required:	Serum
Volume (Metric):	500 ml
Form:	Liquid
Glutamine:	L-Glutamine
Phenol Red Indicator:	Phenol Red
pH Range:	7.0 - 7.4
Concentration:	1 X

储存

Storage conditions: 2-8° C. Protect from light. Shipping conditions: Ambient. Shelf life: 12 months from date of manufacture.

描述

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

The complete formulation is available.

Using MEM α
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

cGMP manufacturing and quality system
MEM α is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical MEM α product made in our Scotland facility (22571-020). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.