NUT MIX F-12 W/GLUTAMAX-I 10 X 500ML，31765068，Invitrogen

规格
Classification:	Animal Origin-Free
Culture Environment:	CO2
HEPES Buffer:	No HEPES
Osmolality:	290 - 320 mOsm/kg
Product Line:	Gibco®, GlutaMAX™
Sodium Bicarbonate Buffer:	Sodium Bicarbonate
Sodium Pyruvate Additive:	Sodium Pyruvate
Supplementation Required:	Serum
Volume (Metric):	500 ml
Form:	Liquid
Glutamine:	GlutaMAX™-I
Phenol Red Indicator:	Phenol Red
pH Range:	7.2 - 7.4
Concentration:	1 X

储存

Storage conditions: 2-8° C. Protect from light. Shipping conditions: Ambient. Shelf life: 12 months from date of manufacture.

描述

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco® F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

The complete formulation is available.

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations. Gibco® F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

cGMP Manufacturing and Quality System
Gibco® F-12 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® F-12 product made in our Grand Island, New York facility (31765-092). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Gibco® F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.